NbActiv1™ neuron culture medium is a pre-mixed version of Neurobasal™ + B27™ + Glutamax™.
NbActiv1, the predecessor to our new NbActiv4, Neurobasal and B27 was developed in 1993 by our founder Greg Brewer,(PM:8377226). It is a serum-free medium optimized for neuron survival after 4 days of growth. Many neuron types can be cultured in NbActiv1, (PM:8600300). Glutamax even replaces the usual glutamine.
Now the convenience of the customary mixture of these ingredients is available in one easy-to-use premixed solution.
Less risk of introducing contamination.
NbActiv1 should be stored at 4°C for up to 6 months if unopened. It should not be frozen. This medium is designed for use at 37°C in a 5% CO2 incubator. Neurons are subject to less oxidative stress if the oxygen is also regulated at 9%.
NbActiv1 must be shipped overnight.
Click here for our Primary Neuronal Culturing Protocol
Click here for our Microglia Protocol
Please do not hesitate to contact us with questions.