SCAVEGRtm - Mix of 5 Antioxidants Added During Brain Mitochondria Isolation Stimulates Higher Respiratory Rates and Better Preserves Creatine Kinase. Also useful for determining the effects of antioxidants on cultured cells.
When diluted from the 50x stock supplied, SCAVEGR can be introduced into the mitochondrial isolation medium during homogenization, centrifugation and resuspension. Respiration of brain mitochondria and synaptosomes are improved compared to isolations performed in standard mannitol/sucrose isolation media alone. The effect of SCAVEGR antioxidants used during measurement of respiration has not been determined. Let us know how well SCAVEGR works for you.
based on the work of Brewer et al. (2004):
Brewer, G.J. Jones, T.T.. Wallimann, T. and Schlattner, U. (2004) Higher Respiratory Rates and Improved Creatine Stimulation in Brain Mitochondria Isolated with Anti-oxidants. Mitochondrion 4:49-57.
We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSA +/- SCAVEGR™ antioxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a 2-fold higher Vmax and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an of antioxidants mixture.
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