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References

Methods based on:

Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination.
Brewer et al. (1993) J. Neurosci. Res. 35:567-576
PM:8377226

Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month.
Brewer & Price (1996) Neuroreport 7:1509-1512. PM:8856709

Precocious axons and improved survival of rat hippocampal neurons on lysine-alanine polymer substrates.
Brewer GJ, Deshmane S, Ponnusamy E. (1998) J Neurosci Methods. Nov 1;85(1):13-20.
PMID:9874136

NbActiv4 medium for neurons increases synaptic densities and network spike rates on multielectrode arrays compared to Neurobasal/B27.  J. Neurosci. meth. 170:181-187 (PM 18308400)

   

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    • Chyan, Y.J., Poeggeler, B., Omar, R.A., Chain, D.G., Frangione, B., Ghiso, J., and Pappolla, M.A. Potent neuroprotective properties against the Alzheimer beta-amyloid by an endogenous melatonin-related indole structure, indole-3-propionic acid. J.Biol.Chem. 274(31), 21937-21942. 1999. PM:10419516 
    • O'Connor, S.M., Andreadis, J.D., Shaffer, K.M., Ma, W., Pancrazio, J.J., and Stenger, D.A. Immobilization of neural cells in three-dimensional matrices for biosensor applications. Biosensors & Bioelec. 14, 871-881. 2000. PM:10945462
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    • Brewer, G.J., Espinosa, J., and Struble, R.G. Effect of Neuregen nutrient medium on survival of cortical neurons after aspiration lesion in rats. J. Neurosurg. 98:1291-1298. 2003.PM:12816277
    • Dombeck, D.A., Kasischke, K.A., Vishwasrao, H.D., Ingelsson, M., Hymanm B.T., and Webb, W.W. Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy. Proc. Natl. Acad. Sci. USA. 100(12):7081-7086. 2003.PM:12766225
    • Grigston, J. C., Vandongen, H. M., McNamara Ii, J. O., and Vandongen, A. M. Translation of an integral membrane protein in distal dendrites of hippocampal neurons. Eur J Neurosci 21, 1457-1468. 2005. PM:15845074 
    • Kushnareva YE, Wiley SE, Ward MW, Andreyev AY, Murphy AN, (2005) Excitotoxic injury to mitochondria isolated from cultured neurons. J Biol Chem 280:28894-28902.  PMID: 15932874
    • McNamara, J. O., Grigston, J. C., Vandongen, H. M., and Vandongen, A. M. Rapid dendritic transport of TGN38, a putative cargo receptor. Brain Res Mol.Brain Res 127, 68-78. 2004.  PM:15306122 
    • Lakkaraju A, Dubinsky JM, Low WC, Rahman YE (2001) Neurons Are Protected from Excitotoxic Death by p53 Antisense Oligonuceotides Delivered in Anionic Liposomes. J Biol Chem 276: 32000-32007.JBC:32000
    • Dalby B, Cates S, Harris A, Ohki EC, Tilkins ML, Price PJ, Ciccarone VC (2004) Advanced transfection with Lipofectamine 2000 reagentprimary neurons, siRNA, and high-throughput applications. Methods 33: 95-103.PM:15121163 
    • Gerencser AA, Mark KA, Hubbard AE, Divakaruni AS, Mehrabian Z, Nicholls DG, Polster BM. Real-time visualization of cytoplasmic calpain activation and calcium deregulation in acute glutamate excitotoxicity. J Neurochem. 2009 Aug;110(3):990-1004. Epub 2009 May 29. PubMed PMID: 19493161; PubMed Central PMCID: PMC2745075 
    • Wang X, Su B, Lee HG, Li X, Perry G, Smith MA, Zhu X. Impaired balance of mitochondrial fission and fusion in Alzheimer's disease. J Neurosci. 2009 Jul 15;29(28):9090-103. PubMed PMID: 19605646; PubMed Central PMCID: PMC2735241
    • X. Wang, B. Su, S. L. Siedlak, P. I. Moreira, H. Fujioka, Y. Wang, G.

      Casadesus, and X. Zhu. Amyloid-beta overproduction causes abnormal mitochondrial dynamics via differential modulation of mitochondrial fission/fusion proteins. Proc.Natl.Acad.Sci.U.S A 105 (49):19318-19323, 2008. PM:19050078
    • M. Das, J. W. Rumsey, N. Bhargava, M. Stancescu, and J. J. Hickman. A defined long-term in vitro tissue engineered model of neuromuscular junctions. Biomaterials 31 (18):4880-4888, 2010.  PM:20346499
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