Papain Protocol for Cell Dissociation

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BrainBits® Papain Protocol For Cell Dissociation

 Enclosed is one glass vial containing 6 mg sterile papain and one 15 mL
centrifuge tube containing 5 mL of Hibernate® E without Calcium
(BrainBits® HE-Ca 5 ml) for improved cell yield during dissociation.

 Preparations (Room Temperature in a Sterile Hood):

1. The cell dissociation solution is prepared by adding 3 mL of the
    supplied HE-Ca to the 6 mg of sterile papain (BrainBits® PAP 6 mg) for 
    a final working concentration of 2 mg/ml papain.
2. Incubate for 10 minutes at 30ºC to dissolve.
3. Once dissolved, add 2 mL of cell dissociation solution to freshly 
   dissected BrainBits® CNS tissue and incubate for 10 min at 30oC.
   Gently swirl every 5 minutes.
4. Disperse and plate cells as per BrainBits® Primary Neuron Culture
    Protocol. For more information refer to:

 For additional tips please see the FAQs at www.BrainBitsLLC.com.

Hibernate® is a trademark of Life Technologies.

Methods based on Brewer et al. (1993) J. Neurosci. Res. 35:567-576 (PMID:
8377226) and Brewer & Price (1996) Neuroreport 7:1509-1512 (PMID: 8856709).