NbActiv1^tm neuron culture medium is a premixed version of Neurobasal^tm + B27^tm + Glutamax^tm. 

NbActiv1, the predecessor to our new NbActiv4, Neurobasal and B27 were developed in 1993 by our founder and President, Greg Brewer, (PM:8377226).  They are a serum-free medium optimized for hippocampal neuron survival after 4 days of growth.  Other neuron types can also be cultured in NbActiv1.  (PM:8600300)  Glutamax even replaces the usual glutamine.  Now the convenience of the customary mixture of these ingredients is availalbe in one easy-to-use premixed solution.  No thawing.  No pipetting.  Less chance for introducing contamination. 

NbActiv1 should be stored at 4 degrees C for up to 4 months.  It should not be frozen.  This medium is designed for use at 37 degrees C in a 5% CO2 incubator.  Neurons are subject to less oxidative stress if the oxygen is also regulated at 9%. 

One half-medium changes with incubator equilibrated medium should be performed at least once a week, with more frequent changes at cell densities above 300 cells/mm2.