Hibernate®
Hibernate is a nutrient medium for the maintenance of neural tissue or cells in ambient carbon dioxide levels (US Patent 6,180,404). Hibernate is useful for maintaining neuron viability during microscopy and flow cytometry. Hibernate also extends the recording time for patch clamp and sharp electrode physiology.
Other uses of Hibernate include:
- Brain storage during genotyping of transgenic mice.
- Shipping brain tissue to collaborators.
BrainBits is also the sole supplier of Hibernate medium.
Hibernate-E is useful for embryonic tissue, Hibernate-A is useful for adult tissue.
References:
- Brewer & Price (1996) Neuroreport 7:1509-1512. PM:8856709
*View fluorescence spectrum of Hibernate and Low Fluorescence Hibernate.
Composition of Hibernate-E® Medium (μM)
Hibernate-A (see footnote)*
| inorganic salts
|
amino acids
|
| CaCl2 (1800)
|
L-alanine (22)
|
| Fe(NO3)3 (0.25)
|
L-arginine (483)
|
| KCl (5360)
|
L-asparagine (5.5)
|
| MgCl2 (812)
|
L-cysteine (7.7)
|
| NaCl (76000)*
|
glycine (400)
|
| NaHCO3 (880)
|
L-histidine (200)
|
| NaH2PO4 (906)
|
L-isoleucine (802)
|
| ZnSO4 (0.67)
|
L-leucine (802)
|
|
|
L-lysine (798)
|
| other compounds
|
L-methionine (201)
|
| D-glucose (25000)
|
L-phenylalanine (400)
|
| phenol red (23)
|
L-proline (67)
|
| MOPS (10,000)
|
L-serine (400)
|
| sodium pyruvate (227)
|
L-threonine (798)
|
| choline chloride (28)
|
L-tryptophan (78)
|
| i-inositol (40)
|
L-tyrosine (398)
|
|
|
L-valine (803)
|
| vitamins
|
|
| D-Ca pantothenate (8)
|
|
| folic acid (8)
|
|
| niacinamide (30)
|
pH 7.30 +/- 0.05
|
| pyridoxal (20)
|
osmolarity 235 +/- 5 mOsm
|
| riboflavin (1)
|
sterile
|
| thiamine (10)
|
|
| B12 (0.2)
|
*Hibernate-A NaCl (89000)
|
|
|
osmolarity 260 +/- 5 mOsm
|
**Hibernate is a trademark of Life Technologies.