25 cm² Culture Flasks

Live Neurons or Astroglia Plated on 25 cm² Culture Flasks

Introducing our new line of live cultured primary neurons adhered to tissue culture treated 25 cm² culture flasks shipped directly to your laboratory with no loss in cell survival.

  • Dramatically reduce time and costs of your primary culture experiments.
  • Optimized for primary cortical or hippocampal neurons and astroglia.  Other CNS regions available upon request. 
  • Neurons are seeded at 16,000 cells / cm² (approximately 400,000 cells per 25 cm² Culture Flask)
  • Astroglia are seeded at 7,500 cells / cm² (approximately 200,000 cells per 25 cm² Culture Flask) and reach 90% confluency by 10 DIV.
  • Excellent for fluorescent microscopy, transfection, pharmacology, physiology, toxicology experiments, as well as protein expression by Western Blot, and mRNA expression by qPCR.

Other brain regions available upon request.

Neurons or Astroglia are plated on Friday for overnight shipment the following Tuesday unless it is a Federal Holiday or other scheduled company closure date. All orders must be received 24 hours prior to cell plating. Neuron or Astroglia cultures arrive 5 days in culture.

Please note: For cell survival, an immediate media exchange with supplied NbActiv4® (neurons) or NbASTRO (astroglia) culture media is required. (NbActiv1™ culture media is available upon request).

 

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